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Image Search Results
Journal: Frontiers in Plant Science
Article Title: A Unique Sulfotransferase-Involving Strigolactone Biosynthetic Route in Sorghum
doi: 10.3389/fpls.2021.793459
Figure Lengend Snippet: Functional characterization of MAX1 analogs from S. bicolor . (A) Phylogenetic analysis of MAX1 analogs. The phylogenetic tree was reconstructed in MEGA X using the neighbor-joining method based on amino acid sequence. The MAX1 analogs are from dicotyledons and monocotyledons. Species abbreviations: Sb, Sorghum biocolor ; Ml, Miscanthus lutarioriparius ; Zm, Zea mays ; Bd, Brachypodium distachyon ; Os, Oryza sativa ; Amt, Amborella trichopoda ; At, Arabidopsis thaliana ; Ac, Aquilegia coerulea ; Pg, Picea glauca ; Sm, Selaginella moellendorffii . For the accession numbers of proteins, see . UP, Unknown product; ND, no detected. (B) SIM extracted ion chromatogram (EIC) at m/z – = 331.1 (green), 347.1 (purple), and m/z + = 331.1 (orange), 347.1 (blue) of CL-producing E. coli co-cultured with A. thaliana P450 reductase 1 (ATR1)-expressing yeast (i) expressing AtMAX1, (ii–v) expressing SbMAX1a–d, and (vi) standards of OB, 4DO, and 5DS. CLA shows characteristic m/z – = 331.1 ( MW = 332.40, [C 19 H 24 O 5 -H] – = [C 19 H 23 O 5 ] – = 331.1); 18-hydroxy-CLA shows characteristic m/z – = 347.1 and m/z + = 331.1 ( MW = 348.40, [C 19 H 24 O 6 -H] – = [C 19 H 23 O 6 ] – = 347.1, [C 19 H 24 O 6 -H 2 O + H] + = [C 19 H 23 O 5 ] + = 331.1); OB shows characteristic m/z + = 347.1 ( MW = 346.38, [C 19 H 22 O 6 + H] + = [C 19 H 23 O 6 ] + = 347.1); 4DO and 5DS show characteristic m/z + signal ( MW = 330.38, [C 19 H 22 O 5 + H] + = [C 19 H 23 O 5 ] + = 331.1). All the traces are representative of at least three biological replicates for each engineered E. coli - S. cerevisiae consortium. 18-OH-CLA stands for 18-hydroxy-CLA. MW stands for molecular weight. Strain used for analysis: AtMAX1 (ECL/YSL1, ), SbMAX1a-d (ECL/YSL2a–d, ).
Article Snippet: The
Techniques: Functional Assay, Sequencing, Cell Culture, Expressing, Molecular Weight
Journal: Frontiers in Plant Science
Article Title: A Unique Sulfotransferase-Involving Strigolactone Biosynthetic Route in Sorghum
doi: 10.3389/fpls.2021.793459
Figure Lengend Snippet: Functional characterization of LGS1 and analogs using CL-producing microbial consortium expressing SbMAX1a. (A) SIM EIC at m/z – = 331.1 (green), 347.1 (purple), and m/z + = 331.1 (orange), 347.1 (blue) of CL-producing E. coli co-cultured with yeast expressing ATR1, SbMAX1a and (i) empty vector (EV), (ii) LGS1, (iii) LGS1-2, (iv) sulfotransferase (SOT) from Triticum aestivum (TaSOT), (v) SOT from Zea mays (ZmSOT), and (vi) standards of OB, 4DO, and 5DS. All traces are representative of at least three biological replicates for each engineered E. coli - S. cerevisiae consortium. (B) Phylogenetic analysis of LGS1. The phylogenetic tree was reconstructed in MEGA X using the neighbor-joining method based on amino acid sequence. The SOTs are from animals, plants, fungi, and cyanobacteria. For the accession numbers of proteins, see . The sequence of LGS1 is from sorghum WT Shanqui Red, LGS1-2 variation is a reference sequence from NCBI, and is four amino acids (DADD) longer than LGS1, see .
Article Snippet: The
Techniques: Functional Assay, Expressing, Cell Culture, Plasmid Preparation, Sequencing